Ion mobility-high resolution mass spectrometry in doping control analysis. Part II: Comparison of acquisition modes with and without ion mobility
نویسندگان
چکیده
In the second part of this study, a systematic comparison was made between two ion fragmentation acquisition modes, namely data-independent (DIA) and DIA with mobility spectrometry (IMS) technology. These approaches were applied to analysis 192 doping agents in urine. Group I included 102 compounds such as stimulants, diuretics, narcotics, β2-agonists, while II contained 90 steroids, glucocorticoids, hormone metabolic modulators. Important method parameters examined compared, including fragmentation, sensitivity, assignment capability minimum occurrence false positive hits. The results differed number detected fragments when exploring MS/MS spectra. only 13%, 64% substances had higher DIA-IMS mode vs. DIA. terms performance modes without activated IMS dimension identical for about 50% agents. sensitivity IMS, i.e. simple mode, 20–40% remaining Despite reduction 82% from both Groups met required level (MRPL) criteria World Anti-Doping Agency (WADA) applied. Automated data processing is important routine analysis. Therefore, methods optimized evaluated prevalence peak assignments by analysing target at different concentrations urine samples. Overall, significantly misidentified observed II, an almost 2-fold misidentifications compared DIA-IMS. This result highlights benefit reduce rate screening UHPLC-IM-HRMS finally samples administration studies nine Groups. However, limit interferences biological matrix, emphasis needed on adequate settings method.
منابع مشابه
Electrospray ionization high-resolution ion mobility spectrometry-mass spectrometry.
A hybrid atmospheric pressure ion mobility spectrometer is described which exhibits resolving power approaching the diffusion limit for singly and multiply charged ions (over 200 for the most favorable case). Using an electrospray ionization source and a downstream quadrupole mass spectrometer with electron multiplier as detector, this ESI-IMS-MS instrument demonstrates the potential of IMS for...
متن کاملIon Mobility Mass Spectrometry
for more complex analysers for automated sample processing and plant control. The separation time will continue to decrease; in the past there has been limited interest in fast separations but this could change as automated sample processing is developed. Increasing use of coupled techniques such as GC-GC, liquid chromatography-GC, and supercritical Suid chromatography-GC for the separation of ...
متن کاملHigh-resolution ion cyclotron mobility spectrometry.
A novel ion mobility spectrometry instrument incorporating a cyclotron geometry drift tube is presented. The drift tube consists of eight regions, four curved drift tubes and four ion funnels. Packets of ions are propagated around the drift tube by changing the drift field at a frequency that is resonant with the ion's drift time through each region. The approach trims each packet of ions as it...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytica Chimica Acta
سال: 2021
ISSN: ['0003-2670', '1873-4324']
DOI: https://doi.org/10.1016/j.aca.2021.338739